Hyalodiscus subtilis from Santa Cruz, California, USA. Arrangement of 4 diatoms. Prepared by WA Firth. Olympus BHB microscope using 450nm LED light. 63x Leitz Pl Apo NA 1.40 objective, oil immersion. Olympus Aplanat Achromat condenser, oil immersion, brightfield lighting. 2.5x Nikon CF PL photoeyepiece. Monochrome converted Nikon d850 camera. 24 images stacked in Zerene (Pmax). Wider field view of the whole arrangement using a 20x Nikon Plan Apo NA 0.65 objective.